Pacific blue fluorophore

spectral signature of fluorophores with very similar spectra, such as Brilliant Violet 421™ and Pacific Blue™. In lieu of using PMTs, these cytometers have Avalanche Photodiode (APD) detectors, which also enables the detection of fluorophores in the near-IR and infrared wavelengths. Spillover Matrix

The BD Spectrum Viewer depicts the excitation and emission curves of fluorochromes for flow cytometry and fluorochrome compatibility and fluorescent spillover.Pacific Blue, or systematically 3-carboxy-6,8-difluoro-7-hydroxycoumarin, is a fluorophore used in cell biology. Its excitation maximum lies at 401 nm, while its emission maximum is at 452 nm. In contrast to the less acidic 7-hydroxy-3-carboxycoumarin (pKa=7.0), the high acidity of the phenol of Pacific Blue (pKa=3.7) causes its fluorescence to remain very high at neutral pH. 32 blue fluorophore 7-hydroxycoumarin (HC) 2 (Figure 1A, top). 33 In the course of our screening, however, we discovered several 34 structures that are not accepted by W37 point mutants. One of 35 the most interesting examples is Pacific Blue (PB), 7 a 36 fluorophore that differs from HC only in the two fluorineThe Alexa Fluor®, Pacific BlueTM, and Pacific OrangeTM Antibody Labeling Kits provide a convenient means to label small amounts of antibodies with the superior Alexa Fluor® dyes or the 405-nm excitable Pacific BlueTM and Pacific OrangeTM dyes. These kits are optimized for labeling 100 μg of antibody per reaction.Pacific Blue® (Pac Blue, PacBlue) is a blue-emitting organic fluorophore that can be excited by the 405 nm Violet laser and captured with a 450/50 nm bandpass filter. Pacific Blue has an excititaion peak at 401 nm and an emission peak at 452 nm. Spectra Alternative Dyes to Pacific Blue Check to add to spectra viewer Pacific Blue V450 ViaFluor 405Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...eFluor 450 dye is a small-molecule fluorescent dye with spectral properties similar to Pacific Blue dye. It was developed to exhibit equal or better fluorescence intensity when compared to Horizon V500 dye or Pacific Blue dye in multicolor applications. Fluorescence intensity comparison. Human PBMCs stained with Anti-CD45RA ( clone HI100 ... Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...

Pacific Blue, or systematically 3-carboxy-6,8-difluoro-7-hydroxycoumarin, is a fluorophore used in cell biology. Its excitation maximum lies at 401 nm, while its emission maximum is at 452 nm. [2] In contrast to the less acidic 7-hydroxy-3-carboxycoumarin (pKa=7.0), the high acidity of the phenol of Pacific Blue (pKa=3.7) causes its ...Fluorochrome Peak Channels UV Excited Fluors Peak Channel BUV395 UV2 BUV496 UV7 BUV563 UV9 BUV661 UV11 BUV737 UV14 BUV805 UV16 Violet Excited Fluors Peak Channel BV421 V1 Alexa 405, SuperBright 436 V2 eFluor 450, VioBlue, Pacific Blue V3 BV480 V4 eFluor 506 V5 BV510, VioGreen V7 BV570, Pacific Orange V8 BV605, SuperBright 600, Qdot 605 …Sep 9, 2011 · An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin, and yet not recognized by the latter’s ligase (W37V LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, E20G/W37T LplA, that ligates Pacific Blue as ... Welcome to FluoroFinder’s Reagent Directory Directory. Here you can find informational pages on fluorescent dyes in our system, with information on excitation and emission characteristics, optimal laser and filter sets, common applications, and more. We are adding dyes to the directory each day so check back for the latest updates.To export and save the data as an image, click the Export Spectra button. A new window with the data will appear as a png file. Save it by right clicking and select “Save image as” or select “Copy image” to paste into another image editor. This spectra analyzer is designed to display the excitation and emission spectra, filters, and ...Two of the most commonly used fluorophores in flow cytometry, R-phycoerythrin (R-PE) and allophycocyanin (APC), are phycobiliproteins originally derived from algae. Both proteins contain fluorescent phycobilin chromophores embedded into a protein scaffolding which accounts for their large size.We report a new method to quantify the affinity of small molecules for proteins. This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, making this approach …Fluorochrome selection is a key step in designing multi-color antibody panels. The list of available fluorochromes is continuously growing, fitting current needs in clinical flow cytometry to simultaneously use more markers to better define multiple leukocyte subpopulations in a single tube. Several criteria guide fluorochrome selection: i) the ...

Pacific Orange and Pacific Blue antibody labeling kits enable attachment of a violet light-excitable fluorescent label on antibodies (up to 100 µg per reaction). Each kit contains enough dye for five reactions. Product Overview Documents Showing 2 of 2 Save to list Add to cart Recurring order eligibleBV421, V450, Pacific Blue,. Alexa405, eFluor450. BFP. DAPI,. SYTOX Blue. eFluor780,. LD405. Prolifn dye (eFluor, V450). FL10. -‐-‐. 525/40 505 -‐ 545. Krome ...from that of commonly used green fluorophores. Alexa Fluor 405: Excited by 405nm violet laser. Alexa Fluor 405 is an extremely dim dye, for that reason it should be only used for the brightest antigens. It is a derivated of the Cascade Blue dye. Alexa Fluor 405 conjugates show minimal spectral overlap with green fluorophores andInvitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Once on the outer surface of the membrane, PS can be detected by fluorophore-labeled annexin V in a calcium-dependent manner. Figure 1 Jurkat cells (human T-cell lymphocytes) untreated control (panel A) or treated with 10 µM camptothecin for 4 hours (panel ... R-phycoerythrin (R-PE), allophycocyanin (APC), and Pacific Blue dye, as well as an ...

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In the example, if the target fluorophore is the Alexa Fluor 488, using the Blue Laser and the GFP channel, 52.8% of the Alexa Fluor 488 emission spectra is captured. By reviewing the table horizontally and using the Blue Laser and the GFP channel or emission filter, the fluorescence emission from the GFP fluorophore is also captured with Alexa ...Pacific BlueTM 404 456 360,405,407 EviTag TM quantum dots-Lake Placid Blue 470 490 AMCyan 457 491 BD Horizon™ V500 415 500 Cy2® 489 506 488 Chromeo TM 488 488 517 DyLight®488 493 518 Alexa Fluor®488 495 519 488 FAM 494 519 Fluorescein Iso-thiocyanate (FITC) 495 519 488 EviTag TM quantum dots-Adirondack Green 505 520 Chromeo TM 505 505 526 ...441. (in buffer): 1. (in antifade): 2. microscopy, flow cytometry, fluorescence microplate reader. Invitrogen Alexa Fluor 350 dye is a blue-fluorescent dye with moderate photostability, and excitation that matches the 350 nm laser line. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.November 5, 2020. Source: Technische Universität Dresden. Summary: Blue phosphorus, an atomically thin synthetic semiconductor, becomes metallic as soon as it is converted into a double layer ...

Choose fluorophores with emissions having the least spectral overlap. For example, although Brilliant Violet 421™ and Pacific Blue™ do not have exact the same emission spectra, they did have significant overlap, so you should generally avoid using these together.1. View Spectra. Normalized emission curves are plotted into each channel off the ultraviolet, violet, blue, yellow/green, and red lasers of Cytek™ Aurora flow cytometers. You can uncheck and remove lasers as necessary to better match your instrument. To view spectra, simply select a fluorophore from one of the dropdowns and then check or ...405 Blue Brilliant Violet ™ 421 BD Horizon™ V450 VPD450 Pacific Blue™ Green BD Horizon V500 AmCyan Orange Brilliant Violet ™ 605a 375b Blue Hoechst 33342 BD Influx™ 488 Green FITC Alexa Fluor® 488 Yellow PE PI Orange BD Horizon PE-CF594 PE-Texas Red® Red 7-AAD PE-Cy5 PerCP PerCP-Cy5.5 Infrared PE-Cy7 532 or 561 …An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin, and yet not recognized by the latter’s ligase (W37V LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, E20G/W37T LplA, that ligates Pacific Blue as ...Pacific Blue™ RPA-T4 440/40 22.5 AmCyan RPA-T4 525/50 20.2 1,2,3 Fluorochromes listed with the same superscript number are read in the same detector, and thus would not normally be used in combination. Table 2. Common choices for 6-, 8-, and 10-color experiments. 6-color 8-color 10-color FITC or Alexa Fluor® 488 FITC or Alexa Fluor® …Fluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ...* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Fluorochrome selection is a key step in designing multi-color antibody panels. The list of available fluorochromes is continuously growing, fitting current needs in clinical flow cytometry to simultaneously use more markers to better define multiple leukocyte subpopulations in a single tube. Several criteria guide fluorochrome selection: …

Hypoxyprobe Pacific Blue Kit (100 mg pimonidazole HCl plus 1 unit of Rat IgG1 monoclonal antibody conjugated to Pacific Blue fluorophore (HP-Pacific Blue)) Item# hp15-100kit $459.00

PerCP/Fire™ 806 is an innovative and unique flow cytometry fluorophore excited by the 488 nm blue laser that emits beyond 800 nm. It can be used on conventional cytometers with a 780/60 filter, but is optimal for spectral instruments with both blue and yellow/green lasers.The LIVE/DEAD Fixable Red (615) Viability kit for 488 and 561 nm excitation was used to differentially stain a mixture of live (left peak) and heat-treated Jurkat cells (right peak). Cells in (A) were not fixed; cells in (B) were fixed in 3.7% formaldehyde following staining. Samples were analyzed by flow cytometry using 488 nm excitation and ...Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BV421, Pacific Blue™ and BD Horizon™ V450 cannot be used simultaneously. Filter: 431/28 or 450/50441. (in buffer): 1. (in antifade): 2. microscopy, flow cytometry, fluorescence microplate reader. Invitrogen Alexa Fluor 350 dye is a blue-fluorescent dye with moderate photostability, and excitation that matches the 350 nm laser line. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets. Ghost Dye™ Blue 516. Ghost Dye™ Red 710. Ghost Dye™ Red 780. Ghost Dye™ UV 450 ... Pacific Blue. Pacific Orange. PE. PE-Alexa Fluor® 610. PE-Alexa Fluor® 700. PE ...The BD Spectrum Viewer depicts the excitation and emission curves of fluorochromes for flow cytometry and fluorochrome compatibility and fluorescent spillover.The net charge on a dextran depends on the fluorophore and the method of preparation. Molecular Probes prepares most of its dextrans by reacting a water-soluble amino dextran with the ... LysoSensor™ Yellow/Blue† 335, 381 452, 521 Indo‡ 356, 343 466, 408 Fura‡ 364, 340 503, 495 Fluo-4 494 516 Oregon ®Green 488 BAPTA-1 496 524 Calcium ...16 авг. 2005 г. ... If calculations indicate that the protein conjugate is labeled with significantly more than seven moles of fluorophore per mole of IgG antibody, ...Marina Blue and Pacific Blue Dyes. Marina Blue and Pacific Blue dyes, both of ... As compared with the aminocoumarin derivatives, the Cascade Blue fluorophore ...

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Choose fluorophores with emissions having the least spectral overlap. For example, although Brilliant Violet 421™ and Pacific Blue™ do not have exact the same emission spectra, they did have significant overlap, so you should generally avoid using these together. Getting around town can be a hassle, especially if you don’t have your own car. But with Blue Van Shuttle Service, you can get to where you need to go quickly and easily. Here are some of the reasons why Blue Van Shuttle Service is the best...We’re celebrating Asian American and Pacific Islander (AAPI) Heritage Month, which is closely following the wins of both Chloé Zhao’s Nomadland and Lee Isaac Chung’s Minari at the Oscars.Texas Red (and Texas Red-X) Dye. Invitrogen Texas Red dye is a bright red-fluorescent dye with excitation ideally suited to the 561 or 594 nm laser lines. It is commonly conjugated to antibodies and proteins for cellular imaging applications. Texas Red sulfonyl chloride is susceptible to hydrolysis and its low solubility in water may complicate ...Dec 19, 2016 · We report a new method to quantify the affinity of small molecules for proteins. This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, making this approach potentially applicable to a wide range of ... Learn about the features and applications of CF® dyes, Biotium's next generation fluorescent dyes for biological research. Download the CF® dye brochure and explore the wide range of colors and conjugates available.Brilliant Violet dyes, including Brilliant Violet 421 (BV421), are a polymer dye-based technology and compatible with both spectral flow cytometry, as well as traditional flow cytometry. The violet laser has unique channels far from heavily occupied detectors allowing for larger panels. BV421 is excited by the 405 nm violet laser and emits at ...A fluorophore (or fluorochrome ... Green is a fluorescein, red is Rhodamine B, yellow is Rhodamine 6G, blue is quinine, purple is a mixture of quinine and rhodamine 6g. Solutions are about 0.001% concentration in water. ... Pacific Blue: 403 455 406 Maleimide Pacific Orange: 403 551 3-Hydroxyisonicotinaldehyde: 385 525 123Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... ….

Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Brilliant Violet dyes, including Brilliant Violet 421 (BV421), are a polymer dye-based technology and compatible with both spectral flow cytometry, as well as traditional flow cytometry. The violet laser has unique channels far from heavily occupied detectors allowing for larger panels. BV421 is excited by the 405 nm violet laser and emits at ... Aug 2, 2013 · Labeling efficiency was a major consideration in the evaluation of our violet dyes, and comparisons have either been reported between V450 and Pacific Blue or presented between V550 and Pacific Orange (Fig. 3), demonstrating that, for certain antibodies, higher numbers of V450 or V550 dye molecules can be attached compared with the number of ... Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... An Improved Synthesis of the Pacific Blue Fluorophore and Fluorescence-based Studies of Receptor-Ligand Interactions Lee, Molly Marie ( University of Kansas , 2016-12-31 ) Early-stage drug discovery and chemical biology projects often use fluorescence-based assays to obtain information about biological interactions and cellular processes.Labeling efficiency was a major consideration in the evaluation of our violet dyes, and comparisons have either been reported between V450 and Pacific Blue or presented between V550 and Pacific Orange (Fig. 3), demonstrating that, for certain antibodies, higher numbers of V450 or V550 dye molecules can be attached compared with the number of ...A fluorophore (or fluorochrome ... Green is a fluorescein, red is Rhodamine B, yellow is Rhodamine 6G, blue is quinine, purple is a mixture of quinine and rhodamine 6g. Solutions are about 0.001% concentration in water. ... Pacific Blue: 403 455 406 Maleimide Pacific Orange: 403 551 3-Hydroxyisonicotinaldehyde: 385 525 123brightest fluorochromes offered by BD Life Sciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to similar excitation and emission properties, BD Horizon™ BV421 and BD Horizon™ V450 cannot be used simultaneously. • • • • • • 405 nm 450/40 Pacific blue fluorophore, Although its absorption peak is in deep blue, it is excited quite effectively in violet (Fig. 15.5), and before the appearance of the BV series fluorochromes, it was often used as a second “violet-driven” fluorochrome emitting to the right of Pacific Blue even though not particularly brilliant., Every racial or ethnic group has specific health concerns. On this page, you'll find links to issues that affect Native Hawaiians and Pacific Islanders. Every racial or ethnic group has specific health concerns. Differences in the health of..., The Alexa Fluor®-labeled antibodies can be used for multiple applications, including fluorescent microscopy, flow cytometry, Western blotting, ELISA, and indirect FISH. Pacific BlueTM and Pacific OrangeTM are unique dyes designed for use with a violet laser (405 nm) for flow cytometry applications., Dory’s character in “Finding Nemo” is a blue tang fish. Blue tang fish are characterized by their bright blue coloring and yellow tail. These fish are found in the Pacific and Indian Oceans., Alexa Fluor 405 dye. Invitrogen Alexa Fluor 405 dye is a blue-fluorescent dye with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. Alexa Fluor 405 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications., The Cytek Aurora leverages full spectrum technology to provide unprecedented flexibility to scientists, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations ..., Marina Blue and Pacific Blue Dyes. Marina Blue and Pacific Blue dyes, both of ... As compared with the aminocoumarin derivatives, the Cascade Blue fluorophore ..., Pacific Blue, or systematically 3-carboxy-6,8-difluoro-7-hydroxycoumarin, is a fluorophore used in cell biology. [1] Its excitation maximum lies at 401 nm, while its emission maximum is at 452 nm. [2], The traditional blue-green 488 nm laser line produced by argon-ion sources was and remains (via newer solid state sources) the primary laser line for most flow cytometric analysis. The 488 nm line excites fluorescein, a low molecular weight fluorochrome that forms the molecular basis for a wide variety of fluorescent cell tags and physiological ..., Reagent Selection Guide for the Attune Flow Cytometers. The Invitrogen Attune cytometers can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry, to match the panels you are currently running. Multiple fluorescent proteins can be interrogated with the 4-laser version of the Attune Flow Cytometers., Our Fluorophore Equivalency Tool will help you find BioLegend’s equivalent fluorophores compared to other commercially available fluorophores. Simply select the name of a competitor's fluorophore from the dropdown menu to see BioLegend's equivalents. The tool also allows you to preview the excitation and emission spectra of our fluorophores ..., spectral signature of fluorophores with very similar spectra, such as Brilliant Violet 421™ and Pacific Blue™. In lieu of using PMTs, these cytometers have Avalanche Photodiode (APD) detectors, which also enables the detection of fluorophores in the near-IR and infrared wavelengths. Spillover Matrix , The Alexa Fluor®-labeled antibodies can be used for multiple applications, including fluorescent microscopy, flow cytometry, Western blotting, ELISA, and indirect FISH. Pacific BlueTM and Pacific OrangeTM are unique dyes designed for use with a violet laser (405 nm) for flow cytometry applications., Fluorochrome Peak Channels UV Excited Fluors Peak Channel BUV395 UV2 BUV496 UV7 BUV563 UV9 BUV661 UV11 BUV737 UV14 BUV805 UV16 Violet Excited Fluors Peak Channel BV421 V1 Alexa 405, SuperBright 436 V2 eFluor 450, VioBlue, Pacific Blue V3 BV480 V4 eFluor 506 V5 BV510, VioGreen V7 BV570, Pacific Orange V8 BV605, SuperBright 600, Qdot 605 …, Note that the standard Dapi/Hoechst filter set will not work with fluorophores that excite at 405 nm. For instance, Alexa 405 and Pacific Blue are bad choices for widefield scopes with standard Dapi/Hoechst filter sets that excite at 365 nm. , blue fluorophore 7-hydroxycoumarin (HC)2 (Figure 1A, top). In the course of our screening, however, we discovered several structures that are not accepted by W37 point mutants. One of the most interesting examples is Pacific Blue (PB),7 a fluorophore that differs from HC only in the two fluorine atoms at C6 and C8 of the coumarin ring (Figure ..., To export and save the data as an image, click the Export Spectra button. A new window with the data will appear as a png file. Save it by right clicking and select “Save image as” or select “Copy image” to paste into another image editor. This spectra analyzer is designed to display the excitation and emission spectra, filters, and ..., DAPI (4′,6-diamidino-2-phenylindole) DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry, and ..., Try the new and improved version of Full Spectrum Viewer in Cytek® Cloud! Sign Up For A Free Account. The new Full Spectrum Viewer features: More fluorescent tags to select from. Fluorescent tag signatures. Complexity™ Index. Similarity™ Index. Stain Index Reduction. Transfer fluorescent tags to experiment templates., Pacific Blue: 455: 500–600: Pacific Green: 500 : Horizon V500, Brilliant Violet 510, VioGreen, Brilliant Violet 570: eFluor 506: 510: Pacific Orange: 550: 600–700: Super Bright 600: 600 : Brilliant Violet 605, ... Fluorophore staining index …, NovaFluor Blue 510 dye should generally be paired with highly expressed antigens to minimize spectral spillover in complex multicolor panels. With distinct spectral signatures, NovaFluor Blue 510, NovaFluor Blue 530, and NovaFluor Blue 555 dyes can be used together in a flow cytometry panel. The macromolecule-based NovaFluor Blue 510 dye ..., Fluorochrome selection largely depends on the intrinsic characteristics ... (APCH7) were compared for the second detector of the red laser line, and Pacific Blue (PacB) versus Horizon V450 ..., See the characteristics of Pacific Blue dye and the range of antibody conjugates, kits, and other products related to this fluorophore. , Quantification of Small Molecule-Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore. ..., StarBright Blue 810. 477. 802. 4. 750LP. 750LP. A488 (ex max 495, ex max 419) With similar fluorescence properties to FITC, Alexa Fluor 488 is not compatible with FITC or GFP. However, as it is brighter and more photostable, it is a great alternative, especially for intracellular staining and in fluorescence microscopy., Quantification of Small Molecule-Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore. ..., Pacific BlueTM 404 456 360,405,407 EviTag TM quantum dots-Lake Placid Blue 470 490 ... donor and acceptor fluorochrome molecule, placed in close proximity, allowing for energy transfer between the two. The tandem dye is excited at the excitation wavelength of the donor molecule and emits a photon at, Annexin V-Pacific Blue Annexin V-eFluor 450 PO-PRO-1 CellTrace Violet Click-iT Plus EdU Pacific Blue Azurite Cerulean TagBFP ECFP mTurquoise AmCyan VioBlue 510/50 520/35 525/50 530/40 550/40 VL2 512/25 VL2 (V6) 525/50 ... Fluorophore and reagent selection guide for flow cytometry, Note that the standard Dapi/Hoechst filter set will not work with fluorophores that excite at 405 nm. For instance, Alexa 405 and Pacific Blue are bad choices for widefield scopes with standard Dapi/Hoechst filter sets that excite at 365 nm., The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres. FluoSpheres™ Microsphere Specifications: • Label (Ex/Em): Blue-green fluorescent (430/465) • Nominal bead diameter: 1.0 µm. • …, If you’re in the market for a new or used boat, one valuable tool that can help you negotiate the best price is a blue book for boats. Before diving into negotiating a price using a blue book for boats, it’s important to have a good underst..., Cannot be used in combination with Invitrogen product line such as Pacific Blue dye, CellTrace Violet stain, FxCycle Violet stain, BV421 or eFluor 450: ... spillover must be compensated for correctly in order to ensure that the fluorescent signal being measure is due to the fluorochrome of interest and thus the cell population of interest., Filipino budget carrier Cebu Pacific announced it would resume regular flights between Manila and Hanoi from next month. - VnExpress International