Higuchi connors phase solubility techniques

Phase solubility studies. Phase solubility studies of stigmasterol with different concentrations of α-CD were determined by the method proposed by Higuchi and Connors at room temperature. The excess amount of stigmasterol was dissolved in 60 ml of ethanol and then added to 10 ml of double-distilled water containing various concentrations of α ...

Phase Solubility Study Figure No. 3. Phase-solubility profiles and classification of complexes. Higuchi and Connors [22] have classified complexes based their effect on substrate solubility as indicated by phase-solubility profiles (Figure 3). A-type phase-solubility profiles are obtained when the solubility of the substrate (i.e., drug ...18. Higuchi T, Connors KA. Phase solubility techniques. Adv Anal Chem Instrum 1965;4:117–212. 19. Higuchi T, Kristiansen H. Binding specificity between small organic solutes in aqueous Solution, Classification of some solutes into two groups according to binding tendencies. J Pharm Sei 1970;59:1601–8.concentration of CD used (Higuchi & Connors, 1965). The Preparation of Complexes. The ... Phase-solubility techniques. Advances in Analytical Chemistry and.The Higuchi and Connors 30 method was used to classify the phase-solubility profiles. The apparent stability constant ( K 1:1, eq 1 ) and the CE ( eq 2 ) were determined from the slope of the linear phase-solubility diagrams plots of the total drug solubility ([ D t ]) versus total concentration of CD in liquid phase ([CD liq ]) in moles per ...Nowadays the application of cyclodextrin-assisted molecular encapsulation in foods offers many advantages. Cyclodextrins, their derivatives and their cross-linked polymers can all improve the quality of food in storage, remove specific components and stabilize and increase the presence components that are important for a healthy diet.Phase-solubility measurementsPhase-solubility measurements were carried out according to the method of Higuchi and Connors. 14 Excess amount of 5-NI (5 mg) was added to 5 mL of deionized water containing increasing amounts of βCD and DM-βCD (ranging from 0 to 0.010 M).Higuchi, KA Connors. Phase-solubility techniques. Adv Anal Chem Instrum 1965; 4: 117–212. Etodolak’ın Ko-kristalizasyonu: Ko-kristalizasyon Tahmini, Ko-kristal Sentezi, Katı Faz Yapı Aydınlatma Çalışmaları ve In Vitro İlaç Salımı ... Higuchi, KA Connors. Phase-solubility techniques. Adv Anal Chem Instrum 1965; 4: 117–212. Show All References …

Solubility phase diagrams were created to evaluate the molar ratios of GAZ/βCD and GAZ/γCD inclusion complexes and their stability in aqueous solution (Fig. 2).The solubility of GAZ increased linearly with the added concentrations of βCD and γCD and produced an A L solubility phase diagram according to the classification by Higuchi, Connors et al. [12] A-type diagrams are formed when the ...Solubility diagrams exhibit AL type according to the Higuchi and Connors classification [23], showing a linear increase ... Phase-solubility techniques. Adv Anal ...Higuchi a nd K. A. Connors, “Phase-solubility techniques, ” Advanc es in Ana lyti cal Ch emi str y and Inst rume ntati on ,v o l . , p p . – , .Using the approach of Higuchi and Connors, phase-solubility studies were performed at increasing concentrations of the three CD. Fig. 2 shows that the solubility of CBD increased linearly with the concentrations of HPβCD and HPγCD which corresponds to the AL type profile (formation of 1:1 inclusion complexes). On the contrary, the phase ...Grant DJW and Higuchi T. Solubility Behavior of Organic Compounds. Wiley, New York, NY, 1990. Google Scholar Higuchi T and Connors KA. Phase-Solubility Techniques. Advan Anal Chem Instr 1965; 4: 117–212. CAS Google Scholar Huang LF and Tong WQ. Abstract. The interaction between ibuprofen and maltodextrins with different dextrose equivalent was studied in solution and solid state in order to investigate the effect on the solubility of ibuprofen and to determine their usefulness in terms of chiral recognition. Apparent binding constants were calculated using nuclear magnetic resonance ...

The K C and CE were calculated from the intrinsic solubility (S 0 ) in the complexing medium and the initial linear slope of the phase solubility diagram (Higuchi and Connors 1965;Loftsson et al ...Nowadays the application of cyclodextrin-assisted molecular encapsulation in foods offers many advantages. Cyclodextrins, their derivatives and their cross-linked polymers can all improve the quality of food in storage, remove specific components and stabilize and increase the presence components that are important for a healthy diet.Phase solubility studies. Solubility measurements were performed in triplicate using the method reported by Higuchi and Connors (Higuchi et al., 1965). An excess amount of ALLO was added to the aqueous solutions of each carrier containing increasing concentrations of the individual carrier (i.e., 0, 1, 5, and 10 % w/v).T. Higuchi and K. A. Connors. Phase solubility techniques. Adv. Anal. Chem. Instrum. 4:117–212 (1965). ... In S. Yalkowsky (ed.), Techniques of Solubilization of Drugs, Marcel Dekker, New York, 1981, pp. 135–149. Google Scholar T. Higuchi and H. Kristiansen. Binding specificity between small organic solutes in aqueous solution ...Solubility diagrams were obtained according to Higuchi and Connors (1965). Excess amounts of sulfamethizole were added to water containing various concentrations of cyclodextrins and the suspensions were shaken at 37°C. ... Phase solubility techniques. Adv. Anal. Chem. Instr., 4 (1965), pp. 117-212. View in Scopus Google Scholar. Job, …Abstract. Solubility is one of the most important physicochemical properties studied during pharmaceutical preformulation. For liquid dosage form development, accurate solubility …

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Types of phase-solubility diagrams according to Higuchi and Connors showing how the total drug solubility changes with increasing CD concentration. A-type diagrams are formed when the drug/CD complex is soluble in the aqueous complexation media and they are usually associated with the water-soluble CD derivatives.Higuchi T, Connors KA. Adv anal chem instrum. Phase-solubility Techniques. 1965;. 4:117-212. 10. Zingone G, Rubessa F. Preformulation study of the inclusion ...method described by Higuchi and Connors (1965). Excess amounts of NB (5 mg) were ... Phase-solubility techniques. Adv Anal Chem Instrum 4: 117–212. Kadri M ...Phase-solubility analysis. Phase-solubility study was carried out according to the method of Higuchi and Connors . Excess amount of repaglinide (approx. 10 mg) was added to 2.5 mL 10 mM phosphate buffer (pH 6 and pH 3.5) containing increasing amounts of Captisol ® (10 −3 –10 −1 M) and shaken at 25 ± 0.5 °C.

The effect of L-arginine was investigated on complexation efficiency of β-cyclodextrin towards Epalrestat in an aqueous media through phase solubility according to Higuchi and Connors. Phase ...where [D/CD] is the concentration of dissolved drug-cyclodextrin complex, [CD] is the concentration of dissolved free cyclodextrin and slope is the slope of the phase solubility profile (Higuchi, Connors, 1965 Higuchi T, Connors AK. Phase solubility techniques. In: Reilly CN, editor. Advances in Analytical Chemistry and Instrumentation.It was found that the solubility of ABPP increased linearly as a func- tion of BCD concentration and showed the fea- tures of an AL type (Higuchi and Connors, 1965). The apparent stability constant, K^.^, of the soluble complex at each temperature was calcu- lated according to the equation of Higuchi and Connors (1965), Eqn 1, where 5 is the …Higuchi T, Connors KA. Phase solubility techniques. Adv Anal Chem Instrum 1965;4:117-212. 10. Asker AF, Whitworth CW. Dissolution of acetyl salicylic acid from acetyl salicylic acid-polyethylene glycol 6000 coprecipitates. Pharmazie 1975;30:530-1. ... T. Higuchi, K.A. Connors. Phase solubility techniques. Adv Anal Chem Instrum, 4 …The Higuchi and Connors 30 method was used to classify the phase-solubility profiles. The apparent stability constant ( K 1:1, eq 1 ) and the CE ( eq 2 ) were determined from the slope of the linear phase-solubility diagrams plots of the total drug solubility ([ D t ]) versus total concentration of CD in liquid phase ([CD liq ]) in moles per ...All the phase-solubility diagrams were of type A except the diagrams of hydrocortisone in γCD and 80/20 γCD/HPγCD solutions were of B S-type according to the Higuchi-Connor classification system. 22 The slopes of the initial linear sections of the diagrams were determined and both the apparent stability constants (K 1:1) and the …Phase solubility test was performed according to the method of Higuchi and Connors . An excess amount of camphor was added to aqueous CD (HPβCD and HPγCD) solutions, and the suspensions were shaken at RT. After equilibrium was achieved at the end of 48 h, the suspensions were filtered with 0.45 μm membrane filter.The most common and widely used method to evaluate the ability of the inclusion complex is the phase solubility studies by Higuchi and Connors (1965). 23 The plot showed that the drug...Mar 10, 2023 · Phase-solubility profiles and classification of drug/CD complexes according to Higuchi and Connors . Figure 2. In general, one drug molecule (D) forms a complex with one CD molecule in pure water, and, if the complex is water-soluble, an A L -type phase-solubility diagram is observed ( Table 1 ).

T K Higuchi. T K Higuchi. Phase solubility techniques ... Phase solubility techniques. T K Higuchi. 1, A Connors. 1. View details (2 authors) Volume: 4, Pages: 212 - 217. …

The phase solubility study was performed in water as per the reported method by Higuchi and Connors . An excess amount of resveratrol was added to a solution containing an increasing concentration of nanosponges. ... Higuchi, T., Connors, K.A.: Phase solubility techniques. In: Advances Analytical Chemistry and Instrumentation, …Higuchi T, Connors K. Phase-solubility techniques. Adv Anal . Chem Instr 1965;4:117-22. ... Microwave Irradiation Technique, a Green Chemistry Approach for Dissolution Enhancement of Lopinavir.Higuchi, KA Connors. Phase-solubility techniques. Adv Anal Chem Instrum 1965; 4: 117–212. Etodolak’ın Ko-kristalizasyonu: Ko-kristalizasyon Tahmini, Ko-kristal Sentezi, Katı Faz Yapı Aydınlatma Çalışmaları ve In Vitro İlaç Salımı ... Higuchi, KA Connors. Phase-solubility techniques. Adv Anal Chem Instrum 1965; 4: 117–212. Show All References …In present work, phase solubility analysis were conducted for studying the solubility of terpineol, a natural flavor compound with antifungal and disinfectant …In a famous publication by Higuchi and Connors, complexes were classified based on the phase solubility profiles derived from the molecular interactions between the host and the guest...[16] T. Higuchi, K. A. Connors, “Phase Solubility Techniques”, Advances in Analytical Chemistry and Instrumentation, 4, 117-150, 1965. [17] T. Higuchi, H. Kristiansen, “Binding specificity between small organic solutes in aqueous solution: Classification of some solutes into two groups according to binding tendencies”, Journal …Mar 10, 2023 · Phase-solubility profiles and classification of drug/CD complexes according to Higuchi and Connors . Figure 2. In general, one drug molecule (D) forms a complex with one CD molecule in pure water, and, if the complex is water-soluble, an A L -type phase-solubility diagram is observed ( Table 1 ). The solubility method was carried out according to the method of Higuchi and Connors (Higuchi and Connors, 1965). The screw capped vials containing samples (10.0–70.0 mg) in excess amounts in aqueous β-CyD solutions (3.0 ml of 0.0–2.0×10 −2 M in 10 mM phosphate buffer, pH 7.4) were shaken at 37°C.

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also calculated whe re possible accordin g to Higuchi-Connors. 8 The methodolog y employed for . ... Connors KA. Phase Solubility Techniques. Advances in Analytical Chemistry and Instrumentation ...The present study was aimed to formulate the solid dispersion incorporated microcapsule to improve the dissolution rate and half life of pioglitazone ...Phase Solubility Study. Phase solubility study was carried out according to the Higuchi and Connors method . For phase solubility study of a binary system, an excess amount of PLDN was added to aqueous solutions containing increasing concentrations of β-CD (3–15 mM). The solutions were kept on rotary shaker for 48 h at 25 °C.Feb 5, 2008 · Phase Solubility Studies. The solubilization ability of CDs can be quantitatively evaluated by the phase solubility method developed by Higuchi and Connors . The phase solubility diagram at 25 °C was obtained by plotting the apparent equilibrium concentrations of the drug against βCD concentrations and is reported in Fig. 1. T. Higuchi, K. A. Connors, “Phase Solubility Techniques,” Advanced Analytical Chemistry of Instrumentation, Vol. 4, 1965, pp. 117-212. has been cited by the following article: TITLE: Binary and Ternary Complexes of Arteether β-CD - Characterization, Molecular Modeling and in Vivo Studies16 Oca 2019 ... bAL: linear phase-solubility diagram and BS: phase-solubility diagram ... (30) Higuchi, T.; Connors, K. A. Phase-solubility techniques. In.Fig. 1 shows the phase solubility diagrams of sulfamethizole with the cyclodextrins used, BCD and HPBCD, at 37°C. The solubility of the drug increased linearly as a function of the CD concentration, a feature of the A L-type complex, showing that water-soluble complexes exit in solution.Phase solubility studies for Cur/Na 2 GA systems were carried out in aqueous systems at different temperatures to calculate the ... (A L-type relationships), suggesting the formation of 1:1 ‘molecular relations’ of associated complexes/micelles (Higuchi & Connors, Citation 1965). Thus, ... Connors KA. (1965). Phase-solubility techniques. In: Reilley CN, ed. …Using the concept proposed by Higuchi and Connors , the solubility diagrams can be classified as the AL type. The observed increase of the drug solubility results from the formation of a drug-cyclodextrin inclusion complex with a stoichiometry of 1:1. ... Higuchi, T.; Connors, K.A. Phase solubility techniques. Adv. Anal. Chem. …Higuchi T, Connors K (1965) Phase solubility techniques. In: Reilly CN (ed) Advances in analytical chemistry instrumentation. Interscience, New York, NY, pp 117–212. Google Scholar Jozefaciuk G, Muranyi A, Fenyvesi E (2001) Effect of cyclodextrins on surface and pore properties of soil clay minerals. Environ. Sci.All experiments were repeated three times. According to the linear part of the phase solubility curve, the stability constant (Kc) was calculated using the Higuchi-Connors equation. (1) K c = S l o p e S 0 × (1-S l o p e) S 0: the solubility of NAR without β-CD. The solubilizing efficiency of β-CD was evaluated by the complexation efficiency ... ….

T. Higuchi and K. A. Connors. Phase solubility techniques. Adv. Anal. Chem. Instrum. 4:117-212 (1965). ... In S. Yalkowsky (ed.), Techniques of Solubilization of Drugs, Marcel Dekker, New York, 1981, pp. 135-149. Google Scholar T. Higuchi and H. Kristiansen. Binding specificity between small organic solutes in aqueous solution ...Phase solubility behavior was studied according to the method proposed by Higuchi and Connors ... Phase solubility techniques. Adv. Anal Chem Instrum 1965; 4: 117 ...Higuchi T, Connoras KA. Phase-solubility techniques, ADV Anal Chem Instrum, 1965, 4, 117-210. has been cited by the following article: Article. Solid Dispersion Incorporated Microcapsules: Predictive Tools for Improve the Half Life and Dissolution Rate of Pioglitazone Hydrochloride.24 Tem 2018 ... This phase-solubility technique was first developed by Higuchi and Connors. ... complex formation solubility, spectral and kinetic techniques. J ...Phase solubility studies. Phase solubility studies were carried out as described by Higuchi & Connors (Higuchi & Connors, 1965). Excess amount of caffeic acid was added to vials containing both CD and ethanol. The concentration of CD varied from 0 to 10 mM and the percentage of ethanol from 0 to 45% v/v.Jun 15, 2019 · Solubility phase diagrams were created to evaluate the molar ratios of GAZ/βCD and GAZ/γCD inclusion complexes and their stability in aqueous solution (Fig. 2).The solubility of GAZ increased linearly with the added concentrations of βCD and γCD and produced an A L solubility phase diagram according to the classification by Higuchi, Connors et al. [12] A-type diagrams are formed when the ... The shake-fl ask method proposed by Higuchi and Connors is the most reliable and widely used solubility measurement method. ... K. A. Connors, Phase-solubility techniques, Adv. Anal. Chem. Instr ...The most common and widely used method to evaluate the ability of the inclusion complex is the phase solubility studies by Higuchi and Connors (1965). 23 The plot showed that the drug...The K C and CE were calculated from the intrinsic solubility (S 0 ) in the complexing medium and the initial linear slope of the phase solubility diagram (Higuchi and Connors 1965;Loftsson et al ... Higuchi connors phase solubility techniques, also calculated whe re possible accordin g to Higuchi-Connors. 8 The methodolog y employed for . ... Connors KA. Phase Solubility Techniques. Advances in Analytical Chemistry and Instrumentation ..., Phase Solubility Studies. The determination of the phase solubility diagram is a widely accepted method for evaluation of the effect of cyclodextrin complexation on the drug solubility. The phase solubility studies were performed by the method reported by Higuchi and Connors . Increasing concentrations of MβCD (0–0.23 M) or …, Phase solubility techniques. T K Higuchi, A Connors. 31 Dec 1964 - Vol. 4, pp 212-217. View 20 related papers. About: The article was published on 1965-01-01 and is currently open access. It has received 2515 citations till now. The article focuses on the topics: Phase (matter) & Solubility., Higuchi and Connors, "Phase-Solubility Techniques,", 1964, Box: 11, Folder: 35. Personal papers of Takeru Higuchi, PP 359. University of Kansas. Kenneth Spencer …, Higuchi, K.A. Connors, Phase solubility techniques Advances in Analytical Chemistry and Instrumentation 4: (1965) 127-212 4. F. Djedaïni, B. Perly, Nuclear Magnetic Resonance of cyclodextrin derivatives and inclusion compounds D. Duchene, New Trends in Cyclodextrins and Derivatives (1992) Editions de Santé Paris Ch. 6 5., After equilibrium was attained, the suspensions were filtered through 0.45 µm cellulose membrane filter, the filtrates diluted with the mobile phase and analyzed by HPLC. The apparent complexation constants for drug/cyclodextrin complexes (K 1:1) were determined according to the phase-solubility method of Higuchi and Connors., The objective of this study was to learn whether or not the pattern recognition methods, such as agglomerative cluster analysis (CA) and principal component analysis (PCA), can be used as supplementary techniques for identification of salicylamide (SAA) inclusion complexes with β-cyclodextrin (β-CD) and 2-hydroxypropyl-β-cyclodextrin (HP …, Phase-solubility profiles and classification of drug/CD complexes according to Higuchi and Connors . Figure 2. In general, one drug molecule (D) forms a complex with one CD molecule in pure water, and, if the complex is water-soluble, an A L -type phase-solubility diagram is observed ( Table 1 )., The effects of βCD and MβCD on the solubility of SMT were studied as follows: an excess of SMT was added to aqueous buffered solutions of pH 2.0, pH 8.0, or water containing different amounts of βCD (0–13.2 mM) or MβCD (0–105 mM for water and 0–84 mM for pH 2.0 and 8.0). The resulting suspensions were sonicated in an ultrasonic …, complexation is the phase so lubili ty method carr ied o ut according to Higuchi and Connors method [14][15] , which examines the e ffec t of nanospo nges on the solubility o f the drug. An, Solubility studies. The phase-solubility diagram corresponding to DPS:βCD system is illustrated in Fig. 5. It was performed according to the method reported by of Higuchi and Connors in order to determine the stability constant of the suggested complex and evaluate its stoichiometry. Measurements were made at 291 nm, which corresponds to the ..., Phase solubility curve of Sbio. Previously, the Higuchi-Connors phase-solubility method was used for assessing the compound stability constant (Higuchi & Connors, 1965). Briefly, extra Sbio was mixed with deionized water (Master-S, Hitech-sciencetool, Shanghai, PR China) containing 0–10 mM γ-CD. Then the suspensions …, Solubility diagrams were obtained by plotting THC concentration versus MeβCD concentration. The association constant K is given by the Eq. (1) of Higuchi and Connors (Higuchi and Connors, 1965). Assuming that the stoichiometry of the inclusion complex is 1:1, the solubility constant is given by the following equation: (1) K 1: 1 = s l o p e [T ..., This was explained by the solubilization theory of Higuchi and Connors. The solubility analysis of the ternary drug/competitor/CyD systems may be particularly useful for determination of the stability constant of a drug whose physicochemical and spectroscopic analyses are difficult, because they can be calculated by monitoring the solubility ..., Phase Solubility Study. Phase solubility study was carried out according to the Higuchi and Connors method . For phase solubility study of a binary system, an excess amount of PLDN was added to aqueous solutions containing increasing concentrations of β-CD (3–15 mM). The solutions were kept on rotary shaker for 48 h at …, The regression analysis (R 2 value 0.9434) of phase solubility data demonstrated A L type curves (Fig. 1 (a)) indicating 1:1 complexation between NCL and HP-β-CD as per Higuchi Connors classification (Higuchi & Connors, 1965). The apparent stability constant of the resultant niclosamide and HP-β-CD complex was found to be 747 M −1., The effects of βCD and MβCD on the solubility of SMT were studied as follows: an excess of SMT was added to aqueous buffered solutions of pH 2.0, pH 8.0, or water containing different amounts of βCD (0–13.2 mM) or MβCD (0–105 mM for water and 0–84 mM for pH 2.0 and 8.0). The resulting suspensions were sonicated in an ultrasonic …, T. Higuchi, K. A. Connors, “Phase Solubility Techniques,” Advanced Analytical Chemistry of Instrumentation, Vol. 4, 1965, pp. 117-212. has been cited by the following …, Solubility phase diagrams were created to evaluate the molar ratios of GAZ/βCD and GAZ/γCD inclusion complexes and their stability in aqueous solution (Fig. 2).The solubility of GAZ increased linearly with the added concentrations of βCD and γCD and produced an A L solubility phase diagram according to the classification by Higuchi, Connors et al. [12] A-type diagrams are formed when the ..., Solubility studies. The phase-solubility diagram corresponding to DPS:βCD system is illustrated in Fig. 5. It was performed according to the method reported by of Higuchi and Connors in order to determine the stability constant of the suggested complex and evaluate its stoichiometry. Measurements were made at 291 nm, which corresponds to the ..., Phase solubility study. The effect of complexation with βCD and HPβCD on the solubility of GA was assessed. The phase solubility diagram was used in order to evaluate the solubility of GA in the presence of CDs according to Higuchi & Connors . The amount of GA was kept in excess and mixed up with different concentrations of βCD (0–16 mM ..., Phase solubility studies in distilled water were conducted according to the method of Higuchi and Connors . Excess amounts of diosmin were added to a series of glass vials containing 10.0 mL of distilled water. ... Higuchi T, Connors KA. Phase solubility techniques. Adv. Anal. Chem. Instrum. 1965; 4:117-212. [Google Scholar] 26. Doile KM ..., The phase solubility diagram for Nag in SBE-β-CD system is shown in Fig. 1. The apparent solubility of Nag increased linearly as a function of SBE-β-CD concentration over the studied concentration range, which could be classified as A L type according to Higuchi & Connors. The slope value was less than one, indicating that the formation of a ..., PHASE-SOLUBILITY TECHNIQUES HIGUCHI TK,CONNORS A. Keyword. 出版时间：. 1965/01/01. 被引量：. 2861. 收藏 引用 批量引用 报错 分享. 全部来源 求助全文. ResearchGate scienceopen.com sid.ir., A stability indicating HPLC assay has been developed in order to investigate the effect of different factors on the stability of omeprazole. These factors included the pH with the tested values ranging from 6–10 and the temperature by monitoring the drug stability at 25, 37 and 40°C. The study was then extended to investigate the effect of ..., Solubility studies used deionized water, and 1 H NMR spectra were of solutions in deuterium oxide (D 2 O, deuterium content 99.8%) from Fluka (Barcelona, Spain). 2.2. Phase solubility studiesSolubility measurements were carried out according to the method of Higuchi and Connors (1965). Excess amounts of clofibrate were added to a series of ..., Abstract. This current work has been conducted mainly to increase solubility and drug release properties for high hydrophobic Dentatin (DEN) by incorporation it into …, [Article] - Phase Solubility Techniques . I'm having a really hard time even finding a link to this article, but everyone cites it: Higuchi, T.; Connors, K. A., 1965. Adv. Anal. Chem. Instrum. 4, 117-212. comment sorted by Best Top New Controversial Q&A Add a …, The plotted diagram displays a straight line with slopes less than 1, suggesting characteristic of Higuchi A L type. This indicate the formation of a 1:1 molecular ratio between the API and the CD. Using the Higuchi–Connors equation, the apparent solubility constant (K C) was determined from Fig. 1, resulting in a value of 122.01 M-1., The phase solubility diagrams of DMY with α-CD, β-CD, γ-CD, HP-β-CD, and DM-β-CD obtained in water at 25 °C are shown in Fig. 1.The aqueous solubility of DMY increased linearly (r 2 ≥ 0.99) with increasing CD concentration at a range of 0–10 mM. All phase diagrams belonged to A L-type according to Higuchi and Connors, suggesting …, T. Higuchi, K. A. Connors, “Phase Solubility Techniques,” Advanced Analytical Chemistry of Instrumentation, Vol. 4, 1965, pp. 117-212. has been cited by the following article: TITLE: Binary and Ternary Complexes of Arteether β-CD - Characterization, Molecular Modeling and in Vivo Studies, Phase-solubility studies. Solubility measurements were performed in triplicate using the method reported by Higuchi and Connors [13]. An excess amount of CAR was added to the aqueous solutions of Soluplus® in water containing increasing concentrations of Soluplus® (0–20% w/v)., The phase solubility analysis described by Higuchi and Connors is the most widely used method to describe inclusion complexation (Brewster and Loftsson 2007; Challa et al. 2005). The excess insoluble drug is placed in vials with a constant volume of aqueous carrier containing several solutions with high concentrations of CD.